Competitiveness and symbiotic efficiency in alfalfa of Rhizobium favelukesii ORY1 strain in which homologous genes of peptidases HrrP and SapA that negatively affect symbiosis were identified

In acid soils, Rhizobium favelukesii strains, known as Oregon-like are a potential risk for alfalfa production given their parasitic behaviour. this study, we isolated five strains (ORY1 to ORY5) from nodules grown in Uruguayan with 99.7% and 100% 16S rRNA gene sequence identity R. type strain of LUP83. The BOX profiles the isolates showed two different patterns, suggesting some diversity among these acid-tolerant isolates. genome analysis ORY1, LPU83, Or191 that they have around 87.5% common coding genes, including symbiotic genes. Moreover, phylogenetic ORY1 genes nifH , nifD nifK nodA nodB nodD were related E. meliloti . We teste competitiveness by inoculating seeds 99:1 1:99 ratios ORY1:: gusA / U143. both treatments, occupied more than 50% nodules, evidencing its high competitiveness. However, aerial biomass treatments was remarkably different, induced efficient essential provide enough N optimal plant growth. These findings support needing areas where inefficient likely be present. Finally, found three encode amino sequences domains M16 peptidases (with homology bacterial hrrP sapA genes), them contiguous located an accessory plasmid, whereas other one chromosomal gene. involved behaviour strain.